Losing my mind over qPCR inconsistency
My qPCR results show wild variation between technical replicates. I must be doing something wrong but for the life of me I can't figure it out. I mix each 20ul reaction via pipetting with a p20 set to 19 before loading, thinking it would be sufficient but things still turn out bad. What else can I do to resolve this??
My qPCR results show wild variation between technical replicates. I must be doing something wrong but for the life of me I can't figure it out. I mix each 20ul reaction via pipetting with a p20 set to 19 before loading, thinking it would be sufficient but things still turn out bad. What else can I do to resolve this??