Silly question

I’m confused right now I have a stock concentration of doxycycline as 2mg/ml and I need 2micro grams/ml. I need a final volume of 10ml. I know the dilution factor is 1000. Thus I need 10 microlitres of doxycycline in 10ml. (2000v=2x10ml —> 2000V=20 —> v=0.01ml or 10microlitres)

What I’m confused on is do I dilute 10microlitres in 10 ml of media or do I dilute 10microlitres in 9.990 ml of media to make up 10ml??

Thank you