Another failed blot ðŸ˜
I think this may be due to too much primary antibody, i did a 1 in 500 dilution overnight at 4 degrees. I haven’t had a problem with mu blocking solution before but I do 5% milk in TBSt.
Some procedure stuff (target 79kda) I ran my gel at 120v for 90 mins Transferred at 85v for 90 mins. Blocked for 2 hours Incubates overnight 1 in 500.
There’s no control band yet …
I think this may be due to too much primary antibody, i did a 1 in 500 dilution overnight at 4 degrees. I haven’t had a problem with mu blocking solution before but I do 5% milk in TBSt.
Some procedure stuff (target 79kda) I ran my gel at 120v for 90 mins Transferred at 85v for 90 mins. Blocked for 2 hours Incubates overnight 1 in 500.
There’s no control band yet …